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Running Your Experiment
How should I dissolve my sgRNA or crRNA/tracrRNA
How can I form a Ribonucleoprotein (RNP) complex between my sgRNA and Cas9 nuclease?
Can you recommend transfection protocols?
How can I measure transfection efficiency and select cells that have been transfected with RNPs?
Can I use Synthego's CRISPRevolution products for gene knock-in experiments?
Can I use Synthego CRISPRevolution RNA kits with my Cas9 stable cell line or with Cas9 mRNA?
I'm using Cpf1 or another nuclease other than S.pyogenes Cas9. How can I order a guide for my nuclease?