Analyze HDR-mediated edits from donors up to 300 bp in length
You can now analyze knock-in edits in addition to knock-outs. Simply specify your donor sequence (up to 300 bp) in addition to your guide sequence and Sanger sequence data and ICE will calculate the efficiency of your edit. Single-base substitutions and small and medium-sized insertions can all be accommodated.
Improved modeling of genotype contributions
Results in fewer spurious genotype calls of low-abundance contributions
We have improved our modeling approach to disfavor genotype contributions that are unlikely to be truly present in analyzed samples. The impact is higher confidence in the remaining contributions and more accurate results overall. You’ll notice fewer contributions overall in the contributions tab, especially those of low abundance (typically under 1-2%). This improvement may result in slightly lower estimates of indel percentage and knockout-score for some analyses.
Enhanced multi-guide analysis support
Supports larger variety of multi-guide edits
Improvements to our modeling of multi-guide events provide more accurate representation of particularly large deletions. Analyses of large edits that previously would have failed will now succeed.
- Clarified table headers
- Appropriate precision in abundance estimates
- More informative error messages
- Bug fixes