Synthego will make a clonal cell line containing a protein coding gene knockout in any human immortalized cell line for you in as few as 10 weeks. We'll edit your gene of interest, derive clones from a highly edited pool of cells and deliver the edited cell clones - with 100% of cell containing the relevant gene knockout.
We design top scoring guides to knock out your gene of interest in your cell line. These guides are synthesized as chemically modified sgRNA and delivered into the cells as ribonucleoprotein complexes with SpCas9 protein. The resulting cell pools are analyzed for total editing efficiency through Sanger sequencing and Inference of CRISPR Edits (ICE) analysis. We then clonally isolate and expand the edited cells and screen clonal cells to identify 100% homogenous and edited populations.