How successful the CRISPR/Cas9 system can perform a specific edit is dependent on a number of factors. One of the largest influences on this process is the guide RNA sequence used. Some sequences can edit cells with very high editing efficiencies, while others perform poorly. How well a guide mediates indel formation is not very well predicted by current “on-target” scores, requiring guide sequences be tested to achieve the highest editing efficiency.
Articles in this section
- Why should I validate my guides?
- What is included in the sgRNA validation service?
- What information is included in the ICE Analysis Report?
- What is ICE?
- What is the KO score?
- How do I interpret the data presented in the ICE Analysis Report?
- What is the best way of utilizing the information from the ICE report?
- How are the on-target and off-target events in the edited cell line analyzed?