Yes, apart from the indels created by non-homologous end joining due to the double-stranded break created by the Cas9 protein at the desired cut site (mediated by the guide RNA), no other components are required nor are inserted into the genome of the cell.
Articles in this section
- What is a Knockout Cell Pool?
- I can do the experiment myself. Why should I buy this?
- What do I get with this order of KO Cell Pools?
- What is a pool of KO cells?
- How do you select for the edited population?
- Do I need to design the guide RNA or target sequences?
- Is there any minimum or guaranteed editing efficiency for these cell pools?
- What are the conditions on the guarantee?
- Can you knockout non-coding genes?
- How do I get my cells to you?