Check out our great blog post detailing all you may need to know about ICE.
ICE determines the percentage of your cell population that has undergone editing as well as the specific indel contributions that make up the edited population. This information can be used in different ways:
- To identify the genotype of the cells that may contribute to a phenotype.
- To calculate the number of colonies that may be necessary to isolate functional knockout clones. In this example, the pool of cells has an ICE score of 97, this suggests that 97% of the population has an indel. Within that 97%, there will be multiple species of indels, thus ICE can also show you this contribution. In this case, the majority indel is a -2 deletion that is present in 68% of the population. Thus, if you isolated 10 colonies from this population of cells, we would anticipate that seven of the ten colonies would likely contain the -2 deletion. Thus, ICE can help determine the number of colonies you need to isolate and screen to identify a functional knockout clone.