This score is useful for determining if your gene editing experiment has resulted in an efficient knockout of protein function. The Knockout or KO Score assumes that the target of interest lies within a coding sequence and calculates the percentage of frameshift indels that fall out of frame from your intended edit and indels that are large enough (21+bp) to be likely to affect protein function. Check out our great blog post detailing all you may need to know about ICE.
Articles in this section
- Why should I validate my guides?
- What is included in the sgRNA validation service?
- What information is included in the ICE Analysis Report?
- What is ICE?
- What is the KO score?
- How do I interpret the data presented in the ICE Analysis Report?
- What is the best way of utilizing the information from the ICE report?
- How are the on-target and off-target events in the edited cell line analyzed?