The on-target events are analyzed by Sanger sequencing followed by indel analysis on Synthego’s Inference of CRISPR Edits (ICE) tool. The report generated by the ICE tool includes information about indel size, location, sequence, and frequency.
Off-target events are predicted in silico by Synthego’s Design Tool and are not tested experimentally. If you would like to have off-targets analyzed for your cells, we offer it for an additional fee. Please contact firstname.lastname@example.org for more information.