Synthego guarantees the knockout cell pool will have a 50% or greater reduction in the protein levels compared to the wildtype cells, as measured by flow cytometry, Western blot or ELISA. Protein analysis should be conducted by the recipient within the first 48 hours after thawing the cell pools, while the indel frequency is still above 50%.
If successful protein knockout is not observed upon testing, Synthego will either replace your knockout cell pool free-of-charge or refund your purchase price. In the event in which knockout of the target gene causes a drastic reduction in cell viability including cell death, we will refund the purchase price of the pool.
This guarantee extends to knockout cell pools that have been purchased from Synthego, and generated by Synthego’s Engineered Cells team.