Synthego will knockout a protein-coding gene in any of 700+ human immortalized cell lines for you in just 4-10 weeks. These cells will contain a heterogeneous pool of edits (indels), with greater than 50% editing efficiency and a guarantee of 50% protein knockout.
To make the cells, we design top scoring guides to knock out your gene of interest in your cell line. These guides are synthesized as chemically modified sgRNA and delivered into the cells as ribonucleoprotein complexes with SpCas9 protein. The resulting cell pools are analyzed for total editing efficiency through Sanger sequencing and Inference of CRISPR Edits (ICE) analysis. We perform quality control checks prior to shipping you the cells.