The Synthego Guide Validation service simplifies your CRISPR editing workflow by providing an editing analysis report on your chosen sgRNA sequences before you use them. Synthego will deliver a modified sgRNA into your selected cell line, extract the edited genomic DNA, PCR amplify the edited site, sequence the site with Sanger sequencing then analyze the data using ICE software.
Along with the shipment of your chosen sgRNA sequences, you will be sent a link to the ICE analysis data. The ICE analysis report contains information about the overall editing efficiency, indel distribution, as well as indel sizes and sequences.
You can find a sample report by clicking "View Example Results".