A single guide RNA (sgRNA) is a chimera of crRNA and tracrRNA that is typically 100 nucleotides in length and consists of three regions:
- A user defined, 17-20nt base-pairing region for specific DNA binding
- A 40nt Cas9 handle hairpin for Cas9 protein binding
- A 40nt long transcription terminator derived from S. pyogenes, that contains hairpin structures that provide stability to the RNA molecule.
Synthego is the only synthetic RNA provider in the industry who can provide full length sgRNA (100-mer) for CRISPR at a practical scale and price point.