Currently, ICE can be used for most standard CRISPR editing that involves the formation of Indels that result from a single CRISPR-Cas9 double-stranded DNA break, as is often used for generating a gene knockout. ICE-D can capture more complex editing outcomes, such as multiplex editing events that utilize several sgRNAs to target and cut a specific locus, which can also be used to generate a more robust gene knockout phenotype. ICE-D can also identify more uncommon editing patterns, such as larger insertions and deletions. Future versions of the ICE tool will be able to analyze more complex CRISPR editing, such as knock-ins and single nucleotide variant (SNV) generation. (These are already available in the source code but not yet available for the web frontend).
Articles in this section
- What is ICE? What is ICE-D?
- What types of CRISPR editing events can ICE and ICE-D analyze?
- What algorithms are used to calculate ICE and ICE-D scores? Which score should I use?
- How does ICE analysis compare to TIDE analysis?
- How does ICE analysis compare to Next Generation Sequencing (NGS) analysis?
- What are some of the benefits of using ICE and ICE-D over TIDE?