The current version of ICE software can analyze indels that result from single or multiplex (up to three sgRNA delivered to cells at once) CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be added to the ICE software tool soon. To request other nucleases or other features and functionalities, please email firstname.lastname@example.org.
The ICE tool also reports a "Knockout score" (KO-score) which represents the proportion of cells that have either a frameshift or 21+ bp indel. This score is a useful measure for those who are interested in understanding how many of the contributing indels are likely to result in a functional KO of the targeted gene.
Future versions of the ICE tool will be able to analyze more complex CRISPR editing, such as knock-ins and single nucleotide variant (SNV) generation. (These are already available in the source code but not yet available for the web frontend).