Inference of CRISPR Editing (ICE) is an open-source, free to use, web-based tool (https://ice.synthego.com) that quickly determines rates of CRISPR-Cas9 editing at a specific, sgRNA directed genomic location within a cell population. Following delivery of CRISPR components, genomic DNA from both edited and unedited (control) populations is PCR-amplified and Sanger sequenced. ICE compares these sequence traces to give a detailed analysis of CRISPR editing.
The current version of ICE software can analyze indels that result from single or multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be added to the ICE software tool soon. To request other nucleases or other features and functionalities, please email email@example.com.