For a chemically modified version of our CRISPRevolution products, we offer 2’-O-methyl analogs and 3’ phosphorothioate internucleotide linkages at the first three 5’ and 3’ terminal RNA residues in the following configuration:
sgRNA: 5′-U*A*A*UUUCACAGCUGCACAUA+Synthego Scaffold…U*U*U*-3′
crRNA: 5′-U*A*A*UUUCACAGCUGCACAUA+Synthego Linker…U*G*G*-3′
tracrRNA: 5′-A*A*A*+Synthego Scaffold…U*U*U*-3′
Chemically modified sequences can provide additional improvements in editing efficiency for particular cell types (e.g. Stem Cells, K562, Prokaryotic) and genomic targets that prove otherwise challenging to edit.
If you’re interested in a chemically modified option, select Modifications from the dropdown menu when inputting your target sequence on the order page. If you would like alternative configurations or modifiers, please contact us.
Click here for more information about chemically modified synthetic gRNA.