The Synthego CRISPR Design tool reports guide RNA sequences that are predicted to be the most effective for knocking out the target gene’s function. The CRISPR Design Tool uses very stringent criteria for selecting and recommending these guides. If the CRISPR Design Tool reports “We found no recommended guides”, then all the potential guides that were evaluated were unable to meet any one or more of the stringent criteria set by Synthego. You can still view all the guides that were found to target the gene, however, these may not necessarily be most effective for generating a gene knockout.
In the "All Guides" page, you can search through different transcript and exons by using the drop-down menu. Because no recommended guides were found, we cannot necessarily come up with a design that will have a guaranteed or certain activity level associated with it. To select a suitable guide from those that are available, we would recommend that you preferentially select those that are: