How can I form a Ribonucleoprotein (RNP) complex between my sgRNA and Cas9 nuclease? Synthego Support June 06, 2017 22:05 Updated Follow Please consult the Synthego Quick Start Guide on the Resources page for RNP formation suggestions. In general, Synthego recommends Cas9:gRNA ratios between 1:3 and 1:9 for RNP formation. Related articles How can I measure transfection efficiency and select cells that have been transfected with RNPs? Can you recommend transfection protocols? How should I dissolve my sgRNA or crRNA/tracrRNA What is sgRNA? What is ICE? What is ICE-D? Comments 0 comments Article is closed for comments.