How can I form a Ribonucleoprotein (RNP) complex between my sgRNA and Cas9 nuclease? Synthego Support September 04, 2018 22:49 Updated Follow Please consult the Synthego Quick Start Guide on the Resources page for RNP formation suggestions. In general, Synthego recommends Cas9:gRNA ratios between 1:3 and 1:9 for RNP formation. Related articles Can you recommend transfection protocols? How should I dissolve my sgRNA or crRNA/tracrRNA How can I measure transfection efficiency and select cells that have been transfected with RNPs? What do I get with an order of KO Cell Clones? How do I use the ICE analysis tool and interpret my results? Comments 0 comments Article is closed for comments.