How can I form a Ribonucleoprotein (RNP) complex between my sgRNA and Cas9 nuclease? Synthego Support May 03, 2018 18:12 Updated Follow Please consult the Synthego Quick Start Guide on the Resources page for RNP formation suggestions. In general, Synthego recommends Cas9:gRNA ratios between 1:3 and 1:9 for RNP formation. Related articles Can you recommend transfection protocols? How can I measure transfection efficiency and select cells that have been transfected with RNPs? How should I dissolve my sgRNA or crRNA/tracrRNA What is sgRNA? How do I use the ICE analysis tool and interpret my results? Comments 0 comments Article is closed for comments.