How should I dissolve my sgRNA or crRNA/tracrRNA Synthego Support April 19, 2019 09:49 Updated Follow Please consult the Synthego Quick Start Guide on the Resources page for best practices related to dissolving, annealing and storing synthetic guide RNAs. Related articles How can I form a Ribonucleoprotein (RNP) complex between my sgRNA and Cas9 nuclease? In what conditions is Synthego synthetic RNA shipped? Can you recommend transfection protocols? What are the differences between custom RNA, sgRNA and cr/tracrRNA? ICE Error: Guide not found in sequence Comments 0 comments Article is closed for comments.