How should I dissolve my sgRNA or crRNA/tracrRNA Synthego Support May 09, 2018 08:38 Updated Follow Please consult the Synthego Quick Start Guide on the Resources page for best practices related to dissolving, annealing and storing synthetic guide RNAs. Related articles How can I form a Ribonucleoprotein (RNP) complex between my sgRNA and Cas9 nuclease? Can you recommend transfection protocols? I'm using Cpf1 or another nuclease other than S.pyogenes Cas9. How can I order a guide for my nuclease? What chemical modification does Synthego offer? What are the advantages? In what conditions is Synthego synthetic RNA shipped? Comments 0 comments Article is closed for comments.