How should I dissolve my sgRNA or crRNA/tracrRNA Synthego Support November 10, 2017 01:35 Updated Follow Please consult the Synthego Quick Start Guide on the Resources page for best practices related to dissolving, annealing and storing synthetic guide RNAs. Related articles How can I form a Ribonucleoprotein (RNP) complex between my sgRNA and Cas9 nuclease? Can you recommend transfection protocols? What chemical modification does Synthego offer? What are the advantages? I'm using Cpf1 or another nuclease other than S.pyogenes Cas9. How can I order a guide for my nuclease? In what conditions is Synthego synthetic RNA shipped? Comments 0 comments Article is closed for comments.