How should I dissolve my sgRNA or crRNA/tracrRNA Synthego Support July 15, 2017 10:46 Updated Follow Please consult the Synthego Quick Start Guide on the Resources page for best practices related to dissolving, annealing and storing synthetic guide RNAs. Related articles Can you recommend transfection protocols? What chemical modification does Synthego offer? What are the advantages? How can I form a Ribonucleoprotein (RNP) complex between my sgRNA and Cas9 nuclease? Why were no recommended guides found? Can I use the CRISPR Design Tool to get primer sequences around my cut site? Comments 0 comments Article is closed for comments.