How should I dissolve my sgRNA or crRNA/tracrRNA Synthego Support September 04, 2018 21:49 Updated Follow Please consult the Synthego Quick Start Guide on the Resources page for best practices related to dissolving, annealing and storing synthetic guide RNAs. Related articles In what conditions is Synthego synthetic RNA shipped? How can I form a Ribonucleoprotein (RNP) complex between my sgRNA and Cas9 nuclease? Can you recommend transfection protocols? What are the differences between custom RNA, sgRNA and cr/tracrRNA? How do I use the ICE analysis tool and interpret my results? Comments 0 comments Article is closed for comments.