How should I dissolve my sgRNA or crRNA/tracrRNA Synthego Support June 20, 2018 00:35 Updated Follow Please consult the Synthego Quick Start Guide on the Resources page for best practices related to dissolving, annealing and storing synthetic guide RNAs. Related articles How can I form a Ribonucleoprotein (RNP) complex between my sgRNA and Cas9 nuclease? Can you recommend transfection protocols? What chemical modification does Synthego offer? What are the advantages? Can I use Synthego CRISPRevolution RNA kits with my Cas9 stable cell line or with Cas9 mRNA? I'm using Cpf1 or another nuclease other than S.pyogenes Cas9. How can I order a guide for my nuclease? Comments 0 comments Article is closed for comments.