Both Synthetic sgRNA and Synthetic Modified sgRNA offer better in vivo stability when duplexed with Cas9 nuclease, compared to plasmid or IVT-derived guide RNAs and crRNA:tracrRNA synthetic systems. Additionally, there is no need to anneal of crRNA:tracrRNA which increases efficiency and reduces lab time. Learn more about Synthego’s Synthetic sgRNA.
Modified sgRNAs provide increased editing efficiency and are recommended for all cell types. They are necessary for modifying the genomes of challenging cell types such as primary or stem cells, plants or prokaryotes or even challenging targets. Target sequences generated by the design tool are identical between modified and unmodified sgRNAs.
Synthetic Modified sgRNA helps researchers achieve superior editing in primary cells, stem cells, challenging cell types (K562, cancer cell lines, plans, prokaryotes), and typically improve editing rates by providing protection against intracellular immune responses that degrade RNA. In addition, chemical modifications provide greater stability and protection from exonucleases to the guide RNA, which may allow for improved editing of challenging genomic targets and cell types. Learn more about Synthego’s Synthetic Modified sgRNA.
Your target sequences will not differ based on Guide Type selection.